HPLC fingerprint of Aquilariae Resinatum Lignum in China / 中草药

ABSTRACT

Objective: To establish an HPLC method for the fingerprint analysis of Aquilariae Resinatum Lignum (ARL), so as to provide evidence for the identification and quality control of ARL. Methods: The analysis was carried out on a Dionex-Acclaim 120 C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile and water-acetic acid (99.50.5) with the flow rate of 0.4 mL/min at 254 nm and the separation was performed at 26℃. The similarity was analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A" and the cluster analysis was performed by SPSS software. Results: The HPLC characteristic fingerprint of ARL has been established. A total of 24 common peaks were characterized, and nine of them were identified by comparing their retention time with reference subslances. The values of similarity evaluation mostly agreed with the result of cluster analysis. Conclusion: It is the first time to establish the HPLC fingerprint of ARL. The method is simple and quick, and reflects the information of chemical composition of ARL comprehensively, which provides the scientific basis for the identification and quality evaluation of ARL.