Phenolic acids from seed coats of Glycine max / 中草药

ABSTRACT

Objective: To study the phenolic acids from the seed coats of Glycine max. Methods: The chemical constituents in the acid water extract from the seed coats of G. max were isolated and purified by chromatographic methods, including macroporous resin, silica gel, ODS, Sephadex LH-20, Toyopearl HW-40, and MCI columns, as well as semi-preparation HPLC. The structures of the isolated compounds were elucidated based on physicochemical properties and spectroscopies, such as ESI-MS, 1H-NMR, and 13C-NMR. Results: Eleven compounds were obtained from the acid water extract and identified as cyanidin-3-O-glucoside (1), methyl caffeate (2), 3-methoxy-4, 5-dihydroxy methyl benzoate (3), protocatechuic acid methyl ester (4), protocatechuic acid ethyl ester (5), 3-methoxy-4-hydroxy benzoic acid (6), 3-methoxy gallic acid (7), dihydroquercetin (8), protocatechuic acid (9), protocateuic acid anhydrite (10), and epicatechin (11). Conclusion: Compounds 2-10 are isolated from the plants in Glycine Wild. for the first time.