ISSR analysis on genetic diversity of Geranium wilfordii from different habitats / 中草药

ABSTRACT

Objective: To analyze the genetic diversity of Geranium wilfordii from different habitats at DNA molecular level and to establish the ISSR-PCR system of G. wilfordii. Methods: Using orthogonal design test combined with the single factor gradient optimization method, the geranium ISSR-PCR system of G. wilfordii was determined. G. wilfordii from 20 germplasmic resources was amplified and analyzed by ISSR-PCR. After translating the amplification results into "0" and "1" matrix, the genetic distances were calculated by Popgene32 and Ntsyspc-2.1 software and the systematic diagram of genetic relationship was clustered by UPGMA method. Results: A total of 207 bands were detected using 15 primers, among which 197 (94.6%) were polymorphic bands. The genetic distances were changed from 0.2018 to 0.9394. The genetic relationship of G. wilfordii was more complex. The 20 samples were divided into two kinds, and the correlation between the most germplasm and its geographic origin had a certain relationship. Conclusion: The significant polymorphism and genetic diversity are observed among G. wilfordii germplasm resources which could provide a wealth of genetic basis for the germplasm high quality.