Studies on chemical constituents in roots and rhizomes of Trillium tschonoskii (II) / 中草药

ABSTRACT

Objective: To investigate the chemical constituents in the roots and rhizomes of Trillium tschonoskii. Methods: The roots and rhizomes of T. tschonoskii were extracted with 70% ethanol and separated by chromatography on polyamide, silica gel, RP-C18, and Sephadex LH-20 columns. Chemical structures were identified by MS, 1D and 2D NMR experiments. Results: Twelve compounds were isolated and identified from the ethyl acetate extract from the roots and rhizomes of T. tschonoskii and n-butanol fractions were identified as β-ecdysone (1), pinnatasterone (2), polypodine B (3), methyl ferulorate (4), regaloside A (5), 4-hydroxy-benzoic acid (6), vanillic aldehyde (7), β-D-glucopyranosyl-(1→4)-O- [α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranoside (8), paris saponin V (9), paris saponin III (10), trillenoside A (11), and trillenoside C (12). Conclusion: Compounds 1, 2, 6, 7, 9, and 10 are isolated from the plants in this genus for the first time, and compounds 3-5, 11, and 12 are isolated from this plant for the first time.