DNA barcode identification of original species in Aesculus Linn / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2593-2599, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-855142
ABSTRACT
Objective:
To screen the DNA barcodes for identifying the species in genus Aesculus Linn.Methods:
The PCR amplification and sequencing efficacy, intraspecific and interspecific variation, and identification efficiency of nuclear ITS, ITS2, chlroplast psbA-trnH, rbcL, and matK sequences from 42 samples of 10 species in genus Aesculus Linn. were investigated. The screened sequences were determined by barcoding gap and NJ tree clustering analysis.Results:
The PCR amplification and sequencing efficacy of psbA-trnH were 100%, the interspecific minimum variation was larger than the intraspecific maximum variation, and psbA-trnH had the highest identification efficacy. The psbA-trnH sequence had less polymerization of intraspecific and interspecific variation. By constructing the NJ tree, thorough verifying the three original plants could be used to separate with other related species.Conclusion:
The psbA-trnH sequence is a powerful, though not perfect, barcode for the identification of species in Aesculus Linn.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2013
Type:
Article
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