Acute toxicity studies on compatibility of "pinellia, trichosanthes, fritillaria, ampelopsis, bletilla attack aconitum" / 中草药

ABSTRACT

Objective: To research the toxicity variation principle of "pinellia, trichosanthes, fritillaria, ampelopsis, bletilla attack aconitum" through studying on the acute toxicity for "pinellia, trichosanthes, fritillaria, ampelopsis, bletilla attack aconitum" in the single herb and anti-drug combination. Methods: Using the median lethal dose (LD50) or maximum tolerate dose (MTD) to evaluate the acute toxicity of single herb, then fix the raw aconitum dose of the compatibility group at LD50, another drug dose increased with the compatibility proportion changed gradually in order to examine the toxicity variation of anti-drug combination with death rate as the index. Results: The LD50 of raw Aconiti Radix (AR) was 4.4 g/kg, the MTD of raw Pinelliae Rhizoma (PR), Trichosanthis Fructus (TF), Fritillariae Thunbergii Bulbus (FTB), Ampelopsis Radix (AR), and Bletillae Rhizoma (BR) was respective 300, 37.8, 272, 167, and 180 g/kg; raw AR and raw PR showed no antagonism phenomenon; raw AR showed the antagonism effect against TF in 41 to 81 while without antagonism in 21-16; the compatibility of raw AR and FTB objected in 61-161 while without antagonism in 11-113; the compatibility of raw AR and AR objected in 3.51-161 while without antagonism in 11-113; the compatibility of raw AR and BR showed no obvious toxicity variation. Conclusion: The ethanol extract of raw AR has obvious acute toxicity; the ethanol extract of raw PR, TF, FTB, AR, and BR has no obvious acute toxicity; under the experimental conditions, the proportion of anti-drug compatibility affects the compatibility of the results.