Simultaneous determination of eight components in Hedan Tablets by UPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2257-2260, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-855181
ABSTRACT
Objective:
To develop a UPLC method for the simultaneous determination of nuciferine, psoralen, isopsoralen, tanshinone IIA, danshensu, sennoside A, sennoside B, and ursolic acid in Hedan Tablets.Methods:
The chromatographic separation was achieved on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with methanol-acetonitrile (4060, A)-0.1% formic acid (B) as mobile phases at the flow rate of 0.6 mL/min for gradient elution 0-4.0 min, 90% B; 4.0-6.0 min, 90%-80% B; 6.0-9.0 min, 80%-70% B; 9.0-10.5 min, 70%-60% B; 10.5-12.0 min, 60%-50% B, 12.0-16.0 min, 50%-10% B; Detection with variable wavelength 0-4.0 min was 215 nm, 4.0-12.0 min was 270 nm, 12.0-16.0 min was 340 nm, and the column temperature was 40°C. Its linear relationship, precision, repeatability, stability, and recoveries were investigated.Results:
The results showed that the eight active components were well separated and showed good linearity. The precision was good and RSD was less than 3.0%. The repeatability was good and RSD was less than 3.0%. The stability was good in 24 h. The average recoveries were between 99.21%-101.91% and RSD was less than 3.0%.Conclusion:
The method is accurate, sensitive, credible, and repeatable. It could be applied to the quality control of Hedan Tablets.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Traditional and Herbal Drugs
Année:
2013
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS