Effect of oroxylin A on blood-brain barrier permeability induced by hypoxic injury in vitro and its mechanism / 中草药

ABSTRACT

Objective: To study the protective effect of oroxylin A on blood-brain barrier (BBB) permeability induced by hypoxic injury and its possible mechanism. Methods: The BBB model in vitro was established by primarily culturing brain microvascular endothelial cells (BMEC), and the hypoxic injury was induced. To observe the changes of BBB permeability, fluorospectrophotometry was used to detect the fluorescence intensity of oroxylin A on fluorescein isothiocyanate (FITC)-dextran in BMEC. The effects of oroxylin A on the mRNA expression of ZO-1 and claudin-1 were assayed by RT-PCR, and the effect on the ZO-1 protein expression was measured by Cell-Based ELISA. Results: Hypoxia significantly increased FITC-dextran permeability, decreased the expression of ZO-1 mRNA and protein, while oroxylin A could antagonize these effects caused by hypoxia. Conclusion: Oroxylin A has a protective effect on BBB permeability induced by hypoxia in vitro, and the mechanism is involved in the regulation of ZO-1 expression.