Promotion of Sambucus williamsii root barks on fracture healing / 中草药

ABSTRACT

Objective: To observe the promotion of Sambucus williamsii (SW) root barks for fracture healing. Methods: Rabbit models with radius fracture were established. The rabbits were divided into control, model, Dieda Pill negative control, and low-, mid-, and high-dose (0.17, 0.34, and 0.68 g/kg) of SW groups. Drugs were added into forage and were given to rabbits 2 d after modeling for 36 d. The effect of SW root barks on serum biochemical indexes and histomorphology of fracture healing was observed. Results: Compared with the model group, a significant increase of alkaline phosphatase (ALP) and acid phosphatase (ACP) was observed in high-and mid-dose SW groups in the initial stage of fracture healing of 12 d after administration (P < 0.05, 0.01). A significent increase of serum bone gla protein (BGP) was observed in the high-and mid-dose SW groups in the middle stage of fracture healing of 24 d after administration (P < 0.05, 0.01). X-ray revealed that deep periosteal reaction density was found, osteotylus was higher and more in the mid-dose SW group, and the density was similar to bone density; Light microscope revealed that trabecular bone appeared in new born bone tissue, and the substance of bone was encapsulated with I-section new born bone tissue. Conclusion: SW root barks have the active fraction for fracture healing by promoting the synthesis and secretion of ALP and BGP of osteoblast, promoting the synthesis of ACP in osteoclast, increasing the quantity of osteoblasts, promoting the absorption of necrotic bone, and speeding up the maturation calcification of bone matrix.