Formula optimization of andrographolide-loaded PLGA microsphere by Box-Behnken design and response surface method / 中草药

ABSTRACT

Objective: To optimize the formula of andrographolide-loaded PLGA microsphere. Methods: The microspheres were prepared by the solvent evaporation method using PLGA as drug carriers. The effects of the concentration of PLGA and PVA, and the oil-water phase ratio were investigated. According to the drug loading amount and encapsulation efficiency, the formula was optimized by Box-Behnken design and response surface method. Results: The optimal formula was as follows: PLGA concentration of 0.20 g/mL, PVA concentration of 18 mg/mL, and oil-water phase ratio of 1:90, respectively. The drug loading amount of the microsphere was (47.21 ± 2.36)% and the encapsulation efficiency was (90.15 ± 3.48)%, which were very close to the predicted values. Conclusion: It is effective and feasible to apply Box-Behnken design and response surface method for the formula optimization of the andrographolide-loaded PLGA microsphere.