Screening for anti-diabetic effective fraction in leaves of Olea europaea / 中草药

ABSTRACT

Objective: To screen the anti-diabetic effective fraction in the leaves of Olea europaea. Methods: Different fractions (Frs. A-F) were prepared using reflux extraction and separation with macroporous resins. α-Amylase system, non-enzymatic glycation system, and alloxan-induced diabetic models of mice were used to evaluate the anti-diabetic activities of the different fractions, respectively. Results: Frs. C-F fractions showed the significant inhibitory activities against α-amylase system and non-enzymatic glycation system. Especially, fraction D showed the best anti-diabetic activity compared with the other fractions, which also displayed a good dose-effect relationship. The anti-diabetic activities of Frs. C and D were both obvious and Fr. D showed more significance. Conclusion: The leaves of O. europaea show the potential anti-diabetic activity and the most effective fraction is ascribed to Fr. D.