Preparation technology of chrysophanol freeze-dried liposomes / 中草药

ABSTRACT

Objective: To establish a method for the preparation of chrysophanol freeze-dried liposomes. Methods: The liposome suspension was prepared by film-ultrasonic dispersion method. The entrapment efficiency (EE) was determined by low-speed centrifugation and the influences of pre-freezing time, drying time, and type, dosage, and adding methods of the protective agents on EE of the freeze-dried liposome were studied. Results: The pre-freezing time was 24 h, the drying time was 24 h, mannitol-sucrose (mass ratio 11) as protective agent was externally added to get uniform particle with good redissolution and the EE was (61.30 ± 2.2)%. Conclusion: The liposome prepared by film-ultrasonic dispersion method is protected by mannitol-sucrose to get uniform particle size with the excellent redissolution and high EE.