Effects of different soaking treatments on seed germination and seedling growth of Platycodon grandiflorum / 中草药

ABSTRACT

Objective: To investigate the effects of different soaking treatments by KNO3, KMnO4, H2O2, GA3, and distilled water for different times on seed germination and seedling growth of Platycodon grandiflorum. Methods: Adopting a double-layer filter paper culture method, the seeds were cultured in the 12 h illumination light incubator at 25 °C, and the germination energy, germination percentage, germination index of the seeds, and the root length and the shoot height of the seedling were recorded. Then the data were analyzed. Results: The best soaking treatment to break seed dormancy, promote the seed germination, and improve the seedling growth of P. grandiflorum was with 0.150 g/L GA3 for 24 h. In addition, another effective soaking treatment is using 0.005 g/mL KNO3 for 12 h. Conclusion: The appropriate soaking reagent and time for the seed germination of P. grandiflorum are obtained, which could provide the guidance for seedling and artificial cultivating P. grandiflorum.