Effects of different treatments on seed germination of Dioscorea nipponica / 中草药

ABSTRACT

Objective: To study the effects of different temperature conditions (10, 15, 20, 25, and 30 °C) and different concentration of gibberellin (GA3) and naphthalene acetic acid (NAA) on the germination responses of Dioscorea nipponica seeds. Methods: The seed germination rate and germination vigor were recorded and analyzed statistically, through setting conditions including cold stratification and soaking treatment with different concentration of GA3/NAA in culture dishes. Results: After sand stratification at 4 °C, the germination percentage and germination vigor of D. nipponica seeds were the highest of 80.00% and 53.33% at 25 °C, respectively. After soaking in 100 mg/L GA3 for 24 h without stratification, the germination percentage and vigor of D. nipponica seeds were 66.65% and 48.35%, respectively; After soaking in 1 mg/L NAA for 12 h, the germination percentage and germination vigor were 58.35% and 3.35%, respectively. The germination process treated with low temperature sand stratification was the fastest. The seeds began to germinate at the day 6, and germinated completely within 12 d. Conclusion: The seeds should germinate at 25 °C after sand stratification at 4 °C for 60 d, or the seeds without stratification would germinate after being treated with 100 mg/L GA3.