Study on effects of extract in Naomaitong Formula on cerebral ischemia-reperfusion model based on NMR metabolomics / 中草药

ABSTRACT

Objective: To clarify the effects of the extract in Naomaitong Formula (ENF) at different doses on cerebral ischemia-reperfusion model of rats and its mechanism in order to optimize the dose group. Methods The focal cerebral ischemia-reperfusion model of Wistar rats was established by an improved middle cerebral artery occlusion (MCAO) method. NMR and principal component analysis (PCA) were applied to analyzing the changes of rat plasma metabolites by ig administration of ENF at 1.5, 3.0, and 6.0 g/kg. And the treatment of ENF for cerebral ischemia-reperfusion model of rats was testified by HE staining with rat brain tissue biopsy. Results PCA and SPSS analyses indicated that the ENF at middle dose of 3.0 g/kg could inhibit the increase of glutamine, taurine, tyrosine, and lactic acid in the plasma of rats, promote the level of lipid, cholesterol, trihydroxy-butyric acid, N-acetyl aspartic acid, acetacetic acid, glutamic acid, and choline, and make them tend to normal. Conclusion Middle dose of ENF could efficiently regulate the metabolic balance after cerebral ischemia-reperfusion injury of rats, so as to play a role of resisting the cerebral ischemia-reperfusion injury of rats.