Effects of solanine on microtubules system of human breast cancer MCF-7 cells / 中草药

ABSTRACT

Objective To investigate the effects of solanine on microtubular system in MCF-7 cell line. Methods Proliferation inhibition of MCF-7 cell line was evaluated by MTT assay. Cell cycle of MCF-7 cells was analyzed and the changes of a-tubulin protein and microtubule-associated protein 2 (MAP-2) protein were detected by flow cytometry. Results The IC50 of MCF-7 cells was 22.08 μg/mL. Solanine could induce MCF-7 cells arrested in S phaseand increase the levels of a-tubulin and MAP-2 in MCF-7 cell line. Conclusion Solanine could inhibit the MCF-7 cell proliferation by increasing a-tubulin and MAP-2 expression and inducing MCF-7 cells arrested in S phase.