Effects of microwave radiation on extraction of epimedin B from Epimedii Folium / 中草药

ABSTRACT

Objective: To investigate the influence of microwave radiation on the extraction of epimedin B from Epimedii Folium. Methods: The effects of extraction solvent, ratio of material to liquor, and duration of microwave radiation on the extraction yield of epimedin B were studied by single factor tests. The influence of microwave radiation on the stability of epimedin B was assessed by UV-Vis spectrophotometeric analysis. The impacts of microwave radiation on leaf sample were observed using paraffin section method. Results: The contributions of ethanol concentration and duration of microwave radiation for the extraction yield of epimedin B are significant, whereas ratio of material to liquor is not a significant factor. Microwave radiation could result in the disruptions of leaf tissues and other parts, but it did not affect the stability of epimedin B. Conclusion: Microwave radiation could facilitate the extraction of epimedin B, which might result from the enhancement of the mass transfer of ethanol solution into leaf tissues and dissolution of epimdin B from the matrix.