Effect of extract from male zooid of Antheraea pernyi on hypoxia-inducible factor-1α expression in tumor-bearing rats after radiotherapy / 中草药

ABSTRACT

Objective: To investigate the effect of the extract from male zooid of Ahtheraea pernyi on the expression of hypoxia-inducible factor-1α (HIF-1α) in tumor-bearing rats after radiotherapy. Methods: W256 tumor model was established by continuous ig administration of the extract from the male zooid of A. pernyi (16.53 mg/kg) for 10 d after conventional radiotherapy. To observe the expression of HIF-1α and vascular endothelial growth factor (VEGF) in tumor tissue by immunohistochemical staining and the HIF-1α mRNA expression by in situ hybridization (ISH). Results: Compared with the model group, immunohistochemistry results showed the expression of HIF-1α and VEGF increased with tendency (P>0.05) in tumor tissue of the rats in radiotherapy group, and the HIF-1α and VEGF expression in the combination group and the extract from the male zooid of A. pernyi group decreased significantly (P0.05) in radiotherapy group compared with the model group. However, the HIF-1α mRNA expression in tumor tissue was lower in both the combined group and the extract from male zooid of A. pernyi group (P<0.05). Conclusion: The extract from male zooid of A. pernyi could down-regulate the HIF-1α expression in tumor tissue after radiotherapy or without radiotherapy.