High performance capillary electrophoresis fingerprint of Corni Fructus pieces / 中草药

ABSTRACT

Objective: To establish a micellar electrokinetic chromatography method with high perfor mance capillary electrophoresis (HPCE) fingerprint of Corni Fructus pieces. Methods: Separation was performed on a fused-silica capillary column (64. 5 cm x 75 μm, effective length 56 cm) with 50 mmol/L borax solution -40 mmol/L SDS- 5% acetonitrile (pH 9. 5) as HPCE buffer. The running voltage was 10 kV, the detection wavelength was 240 nm, and column temperature was 18°C. Taking loganin as reference, the data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples. Results: HPCE Fingerprint was established with nine common peaks. Conclusion The method is accurate, simple, and suitable to the quality control of Corni Fructus.