Effect of resveratrol on apoptosis induction of fibroblast synovial cells and its possible mechanism / 中国药理学通报

ABSTRACT

Aim To explore the effect of resveratrol (Res) on fibroblast synovial cells (FLS) inadjuvant arthritis (AA) rats treated with low concentration of H2O2, and the role of mitochondrial oxidative stress proteins deacetylase 3 (SIRT3) and manganese superoxide dismutase (MnSOD). Methods Twenty SD rats were randomly divided into normal group and model group. The AA model was induced by subcutaneous injection of the complete adjuvant in toes of model group. On 28th day after modeling, the rats were killed and the changes in serum oxidative stress index in two groups were detected. The expression of oxidative stress protein of SIRT3 and MnSOD in synovial tissues of knee joint of AA rats were detected by immunohistochemistry stainning. The effect of different concentrations of H2O2or Res treatment on FLS proliferation was observed by CCK-8 method. The effect of Res on apoptosis and the expression proteins SIRT3 and MnSOD of FLS treated with low H2O2concentration were detected by Hoechst 33258 and Western blot. Results Compared with normal group, the serum of AA rats showed oxidative stress. The expression of SIRT3 and MnSOD increased in synovial tissues of AA model rats. Low concentration of H2O2promoted FLS proliferation, and Res could dose-dependently inhibit proliferation treated with low concentration of H2O2, and increased FLS apoptosis. Moreover, with the increase of Res dose, the expression of SIRT3 and MnSOD protein decreased in FLS treated with low concentration H2O2. Conclusions AA rats are in a state of oxidative stress, low concentration of H2O2can promote FLS growth in AA ras, and Res treatment can inhibit FLS proliferation, promoting FLS apoptosis. The mechanism may be related to the reduction of anti-oxidative stress effect.