Research on estrogen-like protective effect of quercetin on PC12 cells induced by Aβ25.35 and molecular mechanism / 中国药理学通报

ABSTRACT

Aim To investigate the estrogen-like pro- tective effect of quercetin and its molecular mechanism via the establishment of a model of AD in vitro by inducing PC 12 cells with Aβ25.35 Methods Cell viability was detected by MTT assay. The expressions of ERot and ERß were detected by immunofluorescence staining. The expressions of ERct and ERβ, p-Akt, total-Akt, p-GSK-3β and total-GSK-3β related proteins were assessed by Western blot. Results MTT assay showed that 20 \imo\ • L-1 Aßyjs could significantly reduce cell viability after treatment with PC 12 cells for 24 h (P 0. 05). The expression levels of total-Akt and total-GSK-3β protein remained basically unchanged. When the estrogen receptor was inhibited by ICI 182, 780, the cell viability of PC 12 cells and the expression of p-Akt and p-GSK-3β protein were significantly decresed (P < 0 . 0 1) . Conclusion All the results indicate that quercetin has a protective effect on Aβ25.35 induced PC 12 cell injury. The estrogen-like neuroprotective mechanism is able to activate PI3K/Akt/GSK-3β signaling pathway by the mediation of estrogen receptor alpha.