Effect of exopolysaccharide from Trichoderma pseudokoningii on proliferation and apoptosis of human colon cancer cell line HCT116 / 中国药理学通报

ABSTRACT

;Aim To study the effect of exopolysaccharide from Trichoderma pseudokoningii (EPS) on the proliferation and apoptosis of human colon cancer cell line HCT116. Methods The proliferation of HCT116 cells treated with EPS was examined by CCK-8 assay. The effect of EPS on the clone formation of HCT116 cells was detected by crystal violet staining. Cell apoptotic rate was determined by flow cytometry. The changes of mitochondrial membrane potential were observed by JC-1. The expressions of apoptosis-related proteins Bcl-2 and Bax were analyzed by Western blot, and caspase-9, caspase-8 and caspase-3 expressions were detected by the kit. Results EPS dose- and time-dependently inhibited the proliferation of the HCT116 cells in the range of 0 -800 mg • L " 1 . With increase of EPS concentration, the colony-formation ability of HCT116 cells decreased; the proportion of apoptotic cells increased and mitochondrial membrane potential decreased; the expression of Bcl-2 protein decreased, while the expression of Bax protein increased; the ratio of Bcl-2/Bax decreased gradually; caspase-9, caspase-8 and caspase-3 activities significantly increased. Conclusions EPS can inhibit proliferation of HCT116 and induce its apoptosis by up-regulating expression of Bax, caspase-9,caspase-8, caspase-3 and down-regulating the expression of Bcl-2.