Effect of recombinant human nerve growth factor on wound healing in diabetic rats / 中国药理学通报

ABSTRACT

Aim: To investigate the effect of recombinant human nerve growth factor (rhNGF) on refractory wound model in diabetic rats. Methods: Male SD rats were injected intraperitoneally with streptozotocin (60 mg · kg-1) to prepare a diabetes model; scald wound was prepared on the back of rats to establish a model of refractory wound in diabetic rats. The rats were treated with rhNGF(3, 6, 12 μg · kg-1 i. m.) once for 21 days. The therapeutic effect of rhNGF on scald refractory wound in diabetic rats was detected by measurement of the wound area, HE staining and transmission electron microscopy examine. Results: The wound healing rate of diabetic rats was slower than that of normal control group (P <0. 01), and rhNGF significantly reduced the scald wound area of diabetic rats (P < 0. 05); rhNGF could promote the regeneration of fibroblasts and capillaries in the wound; rhNGF significantly improved the arrangement of epithelial cells, cell connection and subepithelial collagen structure in diabetic rats. Conclusion: rhNGF could promote the recovery of refractory scald wound in diabetic rats.