Studies of cytotoxin from guangxi cobra venom on its purification and cytotoxic effects on the nasopharyngeal and other cancer cells / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 61-64, 2002.
Article
de Zh
| WPRIM
| ID: wpr-857427
Bibliothèque responsable:
WPRO
ABSTRACT
AIM: To study the inhibitory effect of the cytotoxin (CTX) from guangxi cobra venom on human nasopharyngeal cancer cell (CNE) and other tumons cells. METHODS: The cytotoxin was isolated and purified from Guangxi cobra venom by successive chromatography on Sephadex G-50 and CM Sepharose CL-6B columns. Its cytotoxic effects and does-effect relationship on human tumors cell lines were examined by MTT assay. RESULTS: The inhibitory effects of CTX CM-5 on CNE, human ovarian carcinoma cell (Ho8990), uterus cervical carcinoma cell (HELA) and lymphoma (YAC) cell lines showed a definite does-effect relationship. The IC50 (48 h in cubation) was 1.84, 2.59, 1.84 and 0.75 mg·L-1 respctively. The inhibitory effects of CTX CM-5 on CNE increased with time. The IC50 (3 h and 24 h cubation) was 4.78 and 1.04 mg·L-1 respctively. CONCLUSION: CTX from Guangxi cobra venom exhibites strong suppressive effect on cultured tumor cells line in vitro.
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WPRIM
langue:
Zh
Texte intégral:
Chinese Pharmacological Bulletin
Année:
2002
Type:
Article