Apoptosis regulated by (-)-epigallocatechin-3-gallate in nasopharyngeal carcinoma cells using apoptosis cDNA expression array / 中国药理学通报

ABSTRACT

AIM: To investigate the mechanism of apoptosis induced by (-)-epigallocatechin-3-gallate (EGCG) and the intervenient effect of EGCG on apoptosis inducted by Epstein-Barr (EB) virus latent membrane protein 1 (LMP1). METHODS: The established doxycycline regulated nasopharyngeal carcinoma cell line pTet-on-LMP1 HNE2 were exposed to 0.6 mg·L-1 Dox and 100 mg·L-1 EGCG for 24 h. Total RNAs were extracted from cells hybridized with the Atlas apoptosis cDNA expression array membrane containing mainly apoptosis related genes. RESULT: EGCG regulated several apoptosis related genes, they were either up- or down-regulated. CONCLUSION: EGCG induced apoptosis might be mediated through some specific genes and signal transduction pathways, and the presence U of EGCG showed some intervenient effect on LMP1 induced cell apoptosis.