Effect of Cimetidine on the Histopathology and the Expression of SOD2 and GPX1 in Low-dose Accumulative Irradiated Beagle Dogs Spleen / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the effect of cimetidine on the histopathology and the expression of SOD2, GPX1 gene and protein in low-dose accumulative irradiated Beagle dogs spleen and to investigate the mechanism of cimetidine′s protective effect on low-dose accumulative irradiation. METHODS: Twenty-four male Beagle dogs were divided into six groups normal control group,model group,positive drug control group and cimetidine groups at the dose of 5.33, 10.67, 21.33 mg•kg-1 respectively. The dogs were irradiated with 60Co-γ-ray at 0.040 8 mGy•min-1 rate for 23 d. Cimitidine was administered intragastrically during irradiation, once a day. Microscope was adopted to observe the pathologic change of spleen and the expression levels of SOD2,GPX1 gene and protein in the spleen tissue of dogs were detected by qRT-PCR and immunohistochemistry techniques. RESULTS: Compared with the model group,cimetidine ameliorated the pathological changes and ultrastructure lesions in the spleen of dogs. Also compared with the model group, the levels of SOD2 and GPX1 protein in cimetidine groups at the dose of 5.33, 10.67, 21.33 mg•kg-1 were higher(P<0.05) and the levels of SOD2 and GPX1 mRNA of the three cimetidine groups were increased significantly(P<0.01). CONCLUSION: Cimetidine can reduce the histological damage. Cimetidine can upregulate the expressions of SOD2 and GPX1 gene and protein and it has good protective effect on the antioxidant system injury.