Effect of Saposhnikovan Sulphate on the Recruitment and Re-education of Macrophages by MCF-7 Cells In Vitro through CCL3 / 中国药学杂志

ABSTRACT

OBJECTIVE: To observe the inhibition of saposhnikovan sulphate obtained by ultrasonic extraction(S-USPS) on human leukemia MCF-7 cells in vitro and the effect of S-USPS on the recruitment and re-education of macrophages by MCF-7 cells. METHODS: S-USPS was obtained from saposknikovan(SPS) by the method of chlorosulfonic acid-pyridine complex. The inhibition of S-USPS and USPS on proliferation of MCF-7 cells was measured by MTT. THP-1-derived macrophages were differentiated in vitro. The effect of S-USPS on the recruitment of macrophages by MCF-7 cells was detected by transwell migration assay. The effects of S-USPS on the expression and secretion of chemokine were detected by qRT-PCR and ELISA. The effects of S-USPS and CCL3 on the expression of inflammatory cytokines were detected by qRT-PCR. RESULTS: The inhibitory rate of S-USPS on the proliferation of MCF-7 was dose-dependent and time-dependent and was higher than that of USPS(P<0.05).S-USPS increased the transcription and secretion of CCL3 by MCF-7 cells. There were higher expressions of IL-1β, IL-6, and TNF-α mRNA and lower expressions of TGF-β, IL-10 mRNA of macrophages in the S-USPS group than in the control group and MCF-7 supernatant group(P<0.05). The promotion of S-USPS on the expressions of IL-1β and TNF-α was inhibited by CCL3 neutralizing antibody(P<0.05). CONCLUSION: There are significant evidences that the saposhnikovan sulphate effectively promotes the recruitment of macrophages by MCF-7 cells in vitro, and re-educates their secretion profile toward M1-type.