Qualities Study of Protamine Sulfate Raw Material from Different Species of Fish / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the qualities of protamine sulfate raw material from different species of fish. METHODS: The identification and determination of protamine sulfate raw material from different fish species were conducted by HPLC, and the composition of amino acids was tested too. Heavy metals was tested by inductively coupled plasma mass spectrometry. The potency of protamine sulfate was measured by biological assay method. RESULTS: At present, the sources of protamine sulfate on the market were mainly salmon and herring. CONCLUSION: This HPLC method can be applied to effectively identify and determine protamine sulfate raw material in order to improve its quality control.