Alkaloids from the Marine-Derived Fungus Aspergillus fumigatus YK-7 and Their Antitumor Activities / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the secondary metabolites of marine-derived fungus Aspergillus fumigatus YK-7. METHODS: The compounds were isolated by several column chromatographic techniques, including silica gel, ODS, Sephadex LH-20 column chromatography, and HPLC, and their structures were identified on the basis of physicochemical properties and spectroscopic analysis. Trypan blue and MTT methods were applied for determining the effects of the compounds on proliferation of cancer cells in vitro. RESULTS: Ten compounds were obtained, and their structures were identified as pseurotin A (1), pseurotin A1(2), 14-norpseurotin A (3), FD-838 (4), demethoxyfumitremorgin C (5), 9β-hydroxyverruculogen TR-2 (6), 6-methoxyspirotryprostatin B (7), spiro[5H, 10H-dipyrrolo[1, 2-a1', 2'-d]pyrazine-2-(3H), 2'-[2H]indole]-3', 5, 10(1'H)-trione (8), terezine D (9), and 14-hydroxyterezine D (10). CONCLUSION: Compounds 3, 6, 7, 9, and 10 are isolated from marine-derived fungus Aspergillus fumigatus for the first time. Compounds 1-4 exhibite moderate antiproliferative activity against selected cancer cell lines in vitro.