Identification of Imperatorin and Its Metabolites in Vitro and in Vivo by HPLC-QTrap-MS Technology / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1352-1358, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-858629
ABSTRACT
OBJECTIVE:
To identify the metabolites of imperatorin in rat urine, feces and bile after oral administration as well as the transformation products of imperatorin after incubation with rat liver microsomes with HPLC-QTrap-MS technology.METHODS:
The combination use of HPLC-QTrap-MS scanning mode including multiple ion monitoring-information dependent acquisition-enhanced product ion (MRM/MIM-IDA-EPI)mode, precursor scan-enhanced resolution-information dependent acquisition-enhanced product ion (PREC-ER-IDA-EPI)mode and enhanced product ion (EPI) mode were performed for the identification of the metabolites. Based on the simultaneous appearance of [M+H]+ and [M+NH4]+ in the spectrum of PREC, the molecular weight could be unambiguously identified. The structures of compounds were then identified by the fragment ions generated from these three modes.RESULTS:
With the HPLC-QTrap-MS method, 32 metabolites in urine sample, 14 metabolites in faces sample, 6 metabolites in bile sample and 17 transformation products from the rat liver microsomes sample were detected.CONCLUSION:
Imperatorin is metabolized mainly in liver and excreted through kidney. The metabolic profiles of imperatorin in vivo and in vitro have good correlation.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Pharmaceutical Journal
Année:
2017
Type:
Article
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