Correlation of blood concentration and drug dose and reducing lipid efficacy of tectorigenin in rabbits / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the correlation of blood concentration and drug dose and reducing lipid efficacy of tectorigenin in atherosclerosis rabbits. METHODS: Forty-eight Japanese white rabbits were randomly divided into six groups, the normal control group, model group, high-, middle-and low-dose (50, 25, 12.5 mg·kg-1·d-1) tectorigenin groups and atorvastatin (5 mg·kg-1· d-1) group. The normal control group was fed with routine forage, and the other groups were fed with high fat forage for the establishment of atherosclerosis model. After 8 weeks, tectorigenin blood concentration was detected by HPLC. After 12 weeks, blood lipids were detected with automatic biochemical analyzer. We sheared the thoracic and abdominal aorta to observe the formation of atherosclerotic plaque and make pathologic examination. RESULTS: Steady-state blood concentration increased with the increase of drug dose, there was a positive relationship (r = 0.969 1) between them, but not the same proportion. After treatment, serum total cholesterol (TC), triglyceride(TG), low density lipoprotein cholesterol (LDL-C) levels were decreased and high density lipoprotein cholesterol (HDL-C) were increased in the tectorigenin each dose group, but the effect was weaker than atorvastatin group. CONCLUSION: Tectorigenin has obvious effect on reducing blood lipid and anti-atherosclerosis in rabbits, and it is related to the blood concentration and the drug dose.