Content determination of 2α, 3α, 24-trihydroxy-12-alkene-28-ursolic acid in the roots of actinidia eriantha benth as an active component by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To isolate and purify 2α, 3α, 24-trihydroxy-12-alkene-28-ursolic acid from the roots of Actinidia eriantha Benth, establish its determination method, and compare the contents in different extracted parts and samples from different sources. METHODS: An HPLC-PDA method was established for the content determination. The contents of 2α, 3α, 24-trihydroxy-12-alkene-28-ursolic acid in the roots of Actinidia eriantha Benth from five different sources and different extracted parts were determined by standard curve method. RESULTS: The content of 2α, 3α, 24-trihydroxy-12-alkene-28-ursolic acid was higher in the samples from Shouning county of Fuzhou city and Yunhe county of Lishui city while lower in those from Yongjia county of Wenzhou city and Lishui city. And in different extracted parts, the content was the highest in dichloromethane part, lower in ethanol part, and the lowest in methanol part. CONCLUSION: The method is stable and accurate with good reproducibility and can be used for the determination of 2α, 3α, 24-trihydroxy-12-alkene-28-ursolic acid in the roots of Actinidia eriantha Benth. The content of 2α, 3α, 24-trihydroxy-12-al-kene-28-ursolic acid is different in Actinidia eriantha Benth samples from different sources, which is the highest in dichloromethane part.