Comparison of HPLC fingerprints of Amomum villosum Lour., amomurn villosum Lour. var. xanthioides T.L. Wu et Senjen and amomum longiligulare T.L. Wu / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the HPLC fingerprints of Amomum villosum Lour., Amomum villosum Lour. var. xanthioides T. L. Wu et Senjen and Amomum longiligulare T. L. Wu and find their differences. METHODS: The samples were extracted with 75% ethanol aqueous and then analysis was carried out on an Agilent ZORBAX SB-Aq C18 column with the mobile phase consisting of methanol (A) and 0.05% formic acid solution (B). Gradient elution (0 min, 5% A; 5 min, 5% A→15% A; 10 min, 15% A→26% A; 20 min, 26% A→40% A; 45 min, 40% A→70% A; 58 min, 70% A→100% A, 63 min, 100% A) was carried out at the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30℃, and the detection wavelength was set at 263 nm. The software "Similarity Evaluation System for Chromatographic Fingerprint of TCMs (Version 2012.0) " was employed to generate the mean chromatogras and carry out the similarity analysis of the samples. SPSS21.0 was employed to carry out the cluster analysis. RESULTS: The HPLC fingerprints of the three varieties were different according to fingerprinting and cluster analysis. Amomum villosum Lour. var. xanthioides T. L. Wu et Senjen was obvilously differernt fromAmomum villosum Lour. and Amomum longiligulare T. L. Wu. There were 25 common peaks in the former HPLC fingerprint and 29 common peaks in the latter. CONCLUSION: The HPLC fingerprints of three kinds of Amomum villasums were set up for the first time and they provide reference for the identification and quality control of Amomum villosum Lour., Amomum villosum Lour. var. xanthioides T. L. Wu et Senjen, and Amomum longiligulare T. L. Wu.