Effects of nitronyl nitroxide on myocardial hypoxia and apoptosis-associated proteins in hypobaric hypoxia mice / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effects of nitronyl nitroxide(HPN) on the myocardial hypoxia- and the expression of apoptosis-associated proteins in hypobaric hypoxia condition mice. METHODS: Sixty BALB/c mice were divided into normal control group, hypoxia model group, acetazolamide group and HPN group randomly. After single intraperitoneal injection of HPN for 30 min, the mice were exposed to a simulated high altitude of 8 000 m for 12 h. After hypoxic exposure, mice were sacrificed and the content of lactate(LD) and lactate dehydrogenase(LDH) activity in heart were determined. HIF-1, VEGF, caspase-3, Bax and Bcl-2 were detected by immunohistochemistry. RESULTS: Compared with normal control group, the LD level and LDH activity in hypoxia model group increased significantly. In addition, the expression of HIF-1α, VEGF, caspase-3 and Bax were increased, Bcl-2 and Bc1-2/Bax ratio was decreased. Pre-treated with HNP the LD level and LDH activity, the expression of HIF-1α, VEGF, caspase-3 and Bax were decreased effectively, the expression of Bcl-2 and Bc1-2/Bax ratio was increased. CONCLUSION: HPN has protective effect on heart injury induced by hypobaric hypoxia in mice. Its mechanism may be related to the amelioration of energy metabolism, alleviation of oxidative stress as well as inhibition of myocardial apoptosis.