Influence of TPGS 1000 and Soluplus on membrane transport of ginsenoside CK in Caco-2 cell monolayer model / 中国药学杂志

ABSTRACT

OBJECTIVE: To examine the effects of TPGS 1000 and Soluplus on the transport of ginsenoside CK in Caco-2 cell model. METHODS: The effects of TPGS 1000 and Soluplus at different concentrations on ginsenoside CK were evaluated by using Caco-2 cell model. The concentration of ginsenoside CK in cell was examined by ultra high pressure liquid chromatography (UPLC) method. The apparent permeability coefficient (Papp) and the efflux ratio were calculated. RESULTS: When the proportion of ginsenoside CK to TPGS 1000 or Soluplus was 11, 13, and 19, the absorption of ginsenoside CK significantly increased. The efflux and efflux ratio both decreased significantly (P<0.05). TPGS 1000 had more significant promotion effect on the transport of ginsenoside CK than the same dose of soluplus at the same ratio (P<0.05). CONCLUSION: In Caco-2 cell model, both TPGS 1000 and Soluplus can significantly promote the absorption of ginsenoside CK.