VP22 enhances the expression and distribution of the tumor suppressor protein PTEN in BT549 / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare a eukaryotic expression vector for cell penetrating peptide fusion protein PTEN-VP22, and to evaluate whether the cell penetrating peptide VP22 increase the expression and distribution of the tumor suppressor protein PTEN in BT549. METHODS: The eukaryotic expression vectors pcDNA3-PTEN and pcDNA3-PTEN-VP22 were constructed and then transferred to BT549. The expression and distribution of PTEN protein and PTEN-VP22 fusion protein were identified by Western blot and immunofluorescence. RESULTS: PTEN protein and PTEN-VP22 fusion protein were successfully expressed in BT549. The expression and distribution of PTEN-VP22 were significantly increased compared with those of PTEN. The distribution of PTEN-VP22 was in a typical VP22 pattern and mainly distributed in nucleus. CONCLUSION: The cell penetrating peptide VP22 enhances the expression and distribution of the tumor suppressor protein PTEN in BT549.