Preparation and standardization of reference standards of melatonin / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the reference standard of melatonin. METHODS: The structure of the reference standard was identified by UV, IR, NMR, and ESI-MS spectrophotometry. The purity was calculated by normalization method and self-contrasted dilution method. Furthermore, loss on drying, residue on ignition, and hygroscopicity were tested. RESULTS: The purity was 99.8% by normalization method and self-contrasted dilution method. COCLUSION The establishment of reference standards of melatonin can efficiently control the addtion of melatonin into lunctional foods.