Indentification of several kinds of post-translational modifications in recombinant protein pharmaceuticals using MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify several kinds of post-translational modifications in recombinant protein pharmaceuticals by MS/MS. METHODS: Firstly the proteins were digested with enzymes, and the obtained peptides were analyzed by UPLC-MS. By MS analysis, peptides with post-translational modifications could be detected, then it was then identified by MS/MS. RESULTS: Using MS/MS, the disulfide bonds, peptides with similar molecular mass, O-glycosylation site and the type of N-linked oligosaccharides were identified. CONCLUSION: MS/MS analvsis can make up for the deficiency of enzyme digestion in some extent, and provide more sufficient evidence for the identification of post-translational modifications.