Preparation of coumarin 6 micelles and uptake and transport study of micelles across epithelial cell monolayer / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the uptake and transport properties of micelles across Madin Darby canine kidney (MD-CK) cells. METHODS: Coumarin 6 loaded PEG-PLA (PEG3000-PLA3000) micelles were prepared by solvent evaporation method, and the HPLC determination method of coumarin 6 was constructed. Competition uptake of blank micelles with coumarin 6 micelles was studied. Temperature and inhibitors effect on transport of micelles across MDCK cell monolayer were studied, the concentration of Ca and EGTA were also studied. RESULTS: Coumarin 6 micelles could be internalized into cells very quickly, and the uptake of micelles could be inhibited by blank micelles. The transport of micelles was affected by temperature and inhibitors, which means that this process is an active and energy-dependent process. The concentration of Ca2+ and EGTA could affect the transport of the micelles, which means micelles could transport across the cell monolayer via paracellular pathway. The amount of transported coumarin 6 micelles was rather limited compared with coumarin 6 micelles added to the apical side. CONCLUSION: Micelles transport across MDCK cell monolayer via both paracellular and transcellular pathway at the same time.