Study of degranulation induced by the chemical composition of polysorbate 80 in a rat mast (RBL-2H3) cell line / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 371-376, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-859813
ABSTRACT
OBJECTIVE:
To study directive effects of eight types of the chemical composition of polysorbate 80 on the degranulation in the RBL-2H3 rat mast cell line and to illuminate the material basis for its pseudoallergy.METHODS:
RBL-2H3 cells were cultured and treated with varying dosage of eight types of the chemical composition of polysorbate 80. And then the amount of the released β-hexosaminidase was detected. MTT assay was used to determine the cytotoxicity of eight types of the chemical composition of polysorbate 80 which causing RBL-2H3 cells degranulation.RESULTS:
PSM, PSD and PIM induced RBL-2H3 cells' degranulation in a concentration-dependent manner. Degranulation was not detected in cells treated with PS and PI. PSTri, PSTetra and PSD weakly induced the degranulation of RBL-2H3 cells in a concentration-dependent manner. PSM, PSD and PIM showed cytotoxicity with concentration-dependent manner, while PS and PI had no cytotoxicity. PSTri, PSTetra and PSD showed low toxicity cytotoxicity in a concentration-dependent manner.CONCLUSION:
PIM is a major source of toxic substances polysorbate 80, the limit of which must be controlled.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Pharmaceutical Journal
Année:
2014
Type:
Article
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