Accumulation dynamics and distribution characters of vitexin in different parts of Vitex negundo var. cannabifolia / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the accumulation dynamics and distribution law of vitexin during the whole year period in different parts of Vitex negundo var.cannabifolia (Sieb. et Zucc.). METHODS: Vitexin was obtained by ultrasonic extraction, and its content was determined by HPLC. Filodoor column (4.6 mm×150 mm, 5 μm) was used. Methanol-water (volume ratio of 4;6) was used as the mobile phase at a flow rate of 1 mL·min-1. The UV detective wavelength was set at 340 nm, injection volume was 5 μL, and the column temperature was set at 35°C. RESULTS: The content of vitexin was low in vitex negundo branches and flowers, and had obvious regularity in different growth periods of Vitex negundo leaves. The content of vitexin was the highest (1.380%-1.465%) in June and July. Along with the blooming and formation of negundo chastetree to their maturation, the content of vitexin in Vitex negundo leaves decreased. Its content was the lowest (0.720%-0.751%) in September and October. The content of vitexin in Vitex negundo seeds increased(0.435%-1.231%)with seed maturation. CONCLUSION: The content of vitexin in different parts of Vitex negundo presents a regular change in different periods. The detection of vitexin in different growth periods provides experimental basis for the selection of best collection phase and medical parts.