The immunomodulatory and the protection of experimental liver injury of Lonicera macranthoides Hand: Mazz on mice / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the immunomodulatory and the protection of experimental liver injury of Lonicera macranthoides Hand Mazz (LHM) which is the main source of Lonicera confusa DC. METHODS: Immunocompromised mice model induced by intraperitoneal injection of cyclophosphamide and in vitro proliferation experiment of its peritoneal macrophages and spleen lymphocytes were used to evaluate the immunomdulatory effect of LHM. The inhibition of LHM on hepatic injury induced by CCl4 or alcohol on mice was measured. RESULTS: The doses of 1.6, 3.2 and 6.4 g · kg-1 of LHM could significantly increased the thymus index, the spleen index, the carbon dissection index and the macrophage phagocytic index of mice (P<0.01), and obviously raised the proliferation ability of peritoneal macrophages and transformation ability of splenic lymphocytes (P<0.01) The doses of 3.2 and 6.4g · kg-1 of LHM could significantly lower the incremental ALT, AST and MDA level (P<0.01), raise the low SOD level in liver (P<0.01) of hepatic injury mice induced by CCl4 or alcohol, and obviously lighten the pathological damage of hepatic tissue. CONCLUSION: The LHM could significantly promote the proliferation and phagocytosis function of immunocytes on immunocompromised mice. Moreover, it has good protection effect on hepatic injury of mice which is induced by CCl4 or alcohol.