Simultaneous determination of five index components in Rehmanniae Radix by HPCE-DAD / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) method for simultaneous determination of catalpol, aucubin, geniposide, acteoside and cinnamic acid in raw and processed Rehmanniae Radix. METHODS: 60 mmol · L-1 sodium borate was used as buffer solution (5% methanol, pH 9.5), uncoated fused silica capillary (64.5 cm × 75 μm, 56 cm of effective length) was used, separation voltage was 20 kV, detection wavelength was 210 nm, column temperature was maintained at 25°C, and sample was injected at 5 kPa × 6 s. RESULTS: Five index components showed good linearity (r > 0.9978) in the ranges of the tested concentrations, the average recoveries of the method were between 97.63%-103.03%. CONCLUSION: The method is simple, accurate and reproducible, and can be used for the quality control of Rehmanniae Radix.