Study on the secondary metabolites of marine-derived fungus Penicillium sacculum / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the secondary metabolites of marine-derived fungus Penicillium sacculum. METHODS: The compounds were separated by several column chromatographic techniques, and their structures were elucidated by means of physico-chemical properties and spectral data. Trypan blue exclusion assay and MTT method were used to test the anti-tumor activities. RESULTS: Ten compounds were isolated from the fermentation broth and mycelium. Their structures were identified as(R)-1, 3-di-hydro-6-methoxy-3-methylisobenzofuran-4, 5-diol(curvulol)(1), griseofulvin(2), 1, 6-dihydroxy-3-methoxy-8-methylxanthone(3), 1-hydroxy-3-methylxanthone(4), 1, 3, 6-trihydroxy-8-methylxanthone(5), 1, 8-dihydroxy-3-methylanthraquinon(6), 1, 3, 5-trihy-droxy-7-methylanthraquinone(7), citreorosein(8), adenine(9) and thymine(10). Among them, compounds 1 and 3 showed respectively potent and moderate growth inhibitory activities against human leukemia HL-60 cells. CONCLUSION: Compounds 1 and 4 were isolated for the first time from the genus of marine-derived fungus. The 1H-NMR and 13C-NMR signals of compound 1 were assigned for the first time. Compounds 1 and 3 have anti-tumor activities. Copyright 2012 by the Chinese Pharmaceutical Association.