Determination of 5-hydroxylansoprazole/lansoprazole sulfone by HPLC-MS/MS in incubation system in vitro / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 49-53, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-860862
ABSTRACT
OBJECTIVE:
To establish a HPLC-MS/MS method for determing 5-hydroxylansoprazole/lansoprazole sulfone, and optimize the incubated conditions for rat liver microsomes.METHODS:
5-Hydroxylansoprazole/lansoprazole were determined by HPLC-MS/MS. Single factor design was used to optimize the incubated conditions and the enzymes kinetics value was evaluated by graphical analysis with Lineweaver-Burk double reciprocal plots.RESULTS:
The linear range of 5-hydroxy lansoprazole and lansoprazole sulphone in liver microsome incubation system were 5.57-2 520 and 5.42-2 480 ng · mL-1, respectively. The optimal incubated conditions were 10 μmol · L-1 lansoprazole, 0.16 mg liver microsomes, and 10 min incubation, respectively.CONCLUSION:
The HPLC-MS/MS method is accurate and suitable for the determination of 5-hydroxy lansoprazole and lansoprazole sulfone in rat liver microsomes. The incubated condition can be applied for study in the drug interaction with lansoprazole.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Pharmaceutical Journal
Année:
2012
Type:
Article
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