Contrast-enhanced ultrasound in evaluation on renal cortex blood perfusion in rat models of hemorrhagic shock / 中国医学影像技术

ABSTRACT

Objective: Contrast-enhanced ultrasound (CEUS) was used to investigate the feasibility of CEUS for evaluation on renal cortex blood perfusion changes in rat models of different degrees hemorrhagic shock (HS). Methods: Twenty-four healthy male SD rats were randomly and equally divided into sham operation group (Sham group), mild hemorrhagic shock group (T1 group), moderate hemorrhagic shock group (T2 group) and severe hemorrhagic shock group (T3 group, each n=6). CEUS was performed after establishment of models to observe renal cortex blood perfusion, and analysis was performed on the time-intensity curve (TIC). Comparative parameters, including peak, time to peak (TTP), AUC and mean transition time (MTT) were analyzed. Venous blood was taken for renal biochemical examination after CEUS, and renal tissue was taken for pathological examination after the rats were killed. Results: TTP, AUC, MTT and urea nitrogen (Urea) in T1 group increased compared with those in Sham group (all P<0.05), but pathological changes were not obvious. TTP, MTT and creatinine (Crea) in T2 group increased than those in Sham group and T1 group (all P<0.05), AUC in T2 group increased than that in Sham group (P<0.05), and pathological changes in T2 group were more damaging compare with T1 group. TTP, AUC, MTT and Crea in T3 group increased than that in the other groups (all P<0.05), and peak in T3 group increased than that in Sham group and T1 group (both P<0.05). Urea in T3 group increased than that in Sham group (P<0.05), and pathological changes were significant. Significant positive correlations were found between TTP, AUC, MTT and Crea (r=0.789, 0.790, 0.800, all P<0.001). Conclusion: CEUS can quantitatively analyze renal cortex blood perfusion changes of rat models caused by hemorrhagic shock. TIC is useful for quantitative analysis, of which TTP, AUC and MTT can be used as sensitivity indexes.