Experimental study of hematoporphyrinmonomethyl ether based PLGA nanoparticles for photoacoustic imaging-guided sonodynamic therapy / 中国医学影像技术

ABSTRACT

Objective To prepare hematoporphyrinmonomethyl ether (HMME) based poly (lactic-co-glycolic acid) (PLGA) nanoparticles, and to investigate their enhancement effect on photoacoustic (PA) imaging and sonodynamic therapy (SDT) efficacy in vitro. Methods HMME-based PLGA (HMME@PLGA) nanoparticles were synthesized using a facile double emulsion strategy. The characteristics and enhanced PA imaging capability were observed in vitro. Upon ultrasound irradiation, the reactive oxygen species (ROS) generated with MDA-MB-231 cells up-taken HMME@PLGA was investigated using flow cytometry. SDT efficacy on cellular level was further investigated. Results The fabricated HMME@PLGA nanoparticles were demonstrated with homogenized size and distribution, and the average diameter was (333.67±17.50)nm. The Zeta potential was (-10.57±1.98)mV. The encapsulation efficiency of HMME in HMME@PLGA was 75.62%, and the drug loading was 2.90%. When incubation with HMME@PLGA nanoparticles (1 mg/ml) for 24 h, the viability of MDA-MB-231 cells was 87.21%. PA signal intensities increased with the increase of HMME@PLGA concentration in vitro. Upon ultrasound irradiation, ROS was produced in MDA-MB-231 cells with the assistance of HMME@PLGA nanoparticles, further leading to cytotoxic effects and cellular death which showed obvious red fluorescence stained with Calcein-AM/PI. Meanwhile, the lysosomes structures of the dead cells disappeared when stained by acridine orange. Conclusion HMME@PLGA nanoparticles are successfully fabricated and can achieve PA imaging-guided SDT.