Study on Intestinal Flora and Expression of NOX1 in Patients With Irritable Bowel Syndrome / 胃肠病学

ABSTRACT

Background: Studies have shown that intestinal flora and nicotinamide-adenosine dinucleotide phosphate oxidase 1 (NOX1) play important roles in the occurrence of chronic visceral pain, however, the roles in irritable bowel syndrome (IBS) are not clear. Aims: To study the correlation of intestinal flora mucosal characteristics with NOX1 expression in patients with IBS. Methods: Colonic mucosa specimens of IBS patients diagnosed by Rome criteria and healthy subjects from July 2017 to December 2017 at Peace Hospital Affiliated to Changzhi Medical College were collected. High-throughput sequencing was used to detect intestinal mucosal flora, and the species diversity and abundance of the intestinal flora were analyzed. The expression of NOX1 in colonic mucosa was determined by immunohistochemistry. Results: The intestinal mucosal flora of IBS group was mainly composed of Bacteroides, Proteobacteria and Firmicutes. There was no significant difference in diversity of flora among IBS-D group, IBS-U group and control group (P>0.05). Compared with the control group, the abundance of Tissierella was significantly increased in the IBS group (H=6.688, P0.05). Conclusions: Compared with healthy subjects, there is no significant change in the diversity of intestinal mucosal flora in IBS patients. The abundance of some flora is changed, and the change of abundance of flora varies in different subtypes. The expression of NOX1 in colonic mucosa of IBS patients is up-regulated, and there was a certain correlation with the abundance of Tissierella.