Effect and Mechanism of Streptococcus gallolyticus Metabolites on Regulating Growth of Colon Cancer Cells / 胃肠病学

ABSTRACT

Background: Streptococcus gallolyticus (SG) may alter the colon microenvironment through its metabolites, and transforming the human body into a high-risk state prone to tumor formation. Aims: To investigate the effect and mechanism of Streptococcus gallolyticus metabolites (SGM) on growth of colon cancer cells. Methods: Human colon cancer cell line SW620 was conventionally cultured, and were divided into blank group (group A), healthy intestinal flora group (group B), Bifidobacterium metabolites group (group C), SGM group (group D) and Bifidobacterium metabolites + SGM group (group E). CCK-8 test was used to detect the proliferation of tumor cell. Cell migration ability was detected by scratch test. The protein expression of β-catenin was determined by Western blotting. The mRNA expression of c-myc was measured by real time PCR. Results: Compared with group A, group B, group C, cell proliferation was significantly increased (P0.05). Conclusions: SG can enhance the proliferation and migration ability of colon cancer cells through its metabolites, which may be through the activating of Wnt/β-catenin signaling pathway. Moreover, Bifidobacterium metabolites cannot inhibit the enhancement of proliferation and migration ability of colon cancer cells by the SGM.