Application of single-cell RNA sequencing in visual system / 中华实验眼科杂志

ABSTRACT

Single-cell RNA sequencing (scRNA-seq) is an emerging technology that observes transcriptomes' differences between individual cells at the cellular level.The basic strategy is to capture single cells and lysis the cells to get mRNA, then amplify them after reversing transcriptions, finally cDNA is used to build sequencing libraries.At present, this technology has been widely used in many subject areas.Visual system includes retina, lateral geniculate nucleus, and visual cortex, etc.It's responsible for the acquisition and processing of visual information and the formation of vision.Visual information accounts for more than 70% of all sensory information.Therefore, the study of the visual system is particularly important.With the rapid development of science and technology, there are more and more research results on scRNA-seq.This technology is gradually becoming an important tool for guiding clinical practice, and has provided a bridge for basic research transforming to clinical research.In this review, we introduce the main technical routes and methods of scRNA-seq and detail its application in visual system.